I want you to write an introduction to my enzyme project, the name of the enzyme is:

I want you to write an introduction to my enzyme project, the name of the enzyme is:

Enzyme: Alkaline phosphatase enzyme

Inhibitor For Inhibition Studies: 10mM Sodium Phosphate stock solution.

what you need to add in the introduction:

Paragraph-1 contains:

General Information about enzymes and the classifications for the specific project:

 In general, what are enzymes? By what mechanism do they make the reactions go faster?

 Which are the 6 different major classes?

 Alkaline phosphate enzyme belongs to which major class?

 What is the Subclass, and which type of reactions the subclass of enzymes catalyze?

 What is the sub-subclass, and which type of reaction for this sub-subclass?

 What is the individual member number for alkaline phosphatase?

 Now write the full E.C. Number for this specific enzyme based on the above descriptions. (find it from Brenda, IUBMB or other databases).

 In general, what types of reactions do the family of alkaline phosphatase enzymes catalyze?

 What are examples of other individual members of this sub-sub class? Give examples and their substrates.

 Are there cofactors for any of these enzymes in the Main class, subclass, or sub-subclass?

 In which organs and tissues the enzyme your worked on, is present in humans?

 Although they are present in different tissues and organs, are they really different enzymes or isozymes? What type of posttranslational modifications cause these differences?

 What is the biological function of this enzyme?

Paragraph 2 contains:

 Objectives: What did you set forth to determine in this experiment? For which enzyme, substrate and inhibitor?

 Write the structural equation for the reaction with the specific substrate that was used in your experiment for the specific enzyme

 What were your hypotheses you set forth to test?

o Did you hypothesize that the enzyme-catalyzed reaction will have a kinetic response, like any other enzyme?

o What was the hypothesis to be able to monitor the reaction? And in your experiment what was chosen for monitoring and why?

o What was the hypothesis with regard to inhibition studies, and expectations regarding Km, Vmax in presence and absence of inhibitor?

o For the specific inhibitor used, what was the hypothesis with regard to the type of inhibition expected? (based on published literature and structural relationship to the substrate).

In the file uploaded, you will find the experiment theory it will be helpful to understand what the experiment about.

references must be in APA style.