C-terminal His-tagged Calcitonin (5 grams) and untagged GLP-1 peptide (10 grams) are accidentally mixed in 1L of 0.5M: Operations Report, SMU

C-terminal His-tagged Calcitonin (5 grams) and untagged GLP-1 peptide (10 grams) are accidentally mixed in 1L of 0.5M imidazole buffer. QC confirmed that there are no other impurities in the mixture. Design a suitable downstream purification scheme to obtain pharmaceutical-grade calcitonin and GLP-1, each in lyophilized form.

Please include the following in your report:

Process flow diagram
Stream table data
Technical drawing and specification sheet of at least 3 selected unit operations
Design calculations to prove the purification will operate as intended
Other aspects selected based on their functioning in the unit

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